Periodic Exposure of Keratinocytes to Cold Physical Plasma: An In Vitro Model for Redox-Related Diseases of the Skin

Figure 4

Transcriptional response of the antioxidant defense system to acute and chronic redox challenge. Transcription of several antioxidative enzymes in HaCaT keratinocytes was measured using microarray technology. HMOX2 and NQO1 were mainly upregulated over three months in contrast to a transient upregulation of HMOX1 during acute stress phases followed by downregulation after chronic stress exposure (a). SOD1, SOD3, and catalase were mainly upregulated (b). GPX1, GPX5, and GPX8 mRNA copy numbers were enhanced whereas those of GPX3 were found to be reduced (c). Expression of several peroxiredoxins was increased after periodic oxidative stress (d). Data are presented as mean + SD of two analyses. Statistical analysis was done using one-way ANOVA with Dunnett corrections for multiple comparisons to untreated, normalized control (, , ).

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