Inhibition of Neutrophil Collagenase/MMP-8 and Gelatinase B/MMP-9 and Protection against Endotoxin Shock

Figure 4

P2 protects Swiss mice from endotoxin shock after intraperitoneal administration. For all the experiments, 35 mg/kg Regasepin 2 via intravenous injection was included as a positive control. (a) A single intravenous injection of 10 mg/kg P2 protected mice from endotoxin shock, whereas two injections of 10 mg/kg P2 at two hours interval decreased the survival rate of the mice. The protective effects of P2 at a dosage of 150 mg/kg via intraperitoneal injection 30 minutes (b), 60 minutes (c), and 90 minutes (d) before intravenous injection of LPS (200 μg per mouse) were shown. Kaplan-Meier survival curves were generated. The results of two parallel and independent experiments were pooled. The details of the animal experiments were tabulated in Tables 3 and 4.

(a)

(b)

(c)

(d)