Stress-Related Gene Expression in Liver Tissues from Laying Hens Housed in Conventional Cage and Cage-Free Systems in the Tropics

Table 1

Sequences of primers used for the gene expression of the interest genes.

Genes			Primer sequence 5′-3′	Amplicon size (pb)	References
Acute phase proteins	AGP	F	TGG​GTG​TAC​ATC​ATG​GGT​GC	80	Authors
		R	AGG​GTG​AAG​GTC​GCG​TAC​TT
	CRP	F	ATA​CGT​CGC​CTT​CCA​CAT​CC	150
		R	TCGTTGCCCACCACGTA
Oxidative stress	NOX4	F	TGT​ACC​GCT​ACA​TCC​GCA​G	159
		R	GGC​TTT​CCA​GTC​CAG​ACA​CT
	SOD1	F	CGG​GCC​AGT​AAA​GGT​TAC​TGG​AA	83
		R	TGT​TGT​CTC​CAA​ATT​CAT​GCA​CAT​G
	CAT	F	TCG​TCT​CTT​TGC​GTA​TCC​TGA	80
		R	TGT​AGG​GGC​AAT​TCA​CAG​GA
	GPX1	F	CAA​CGG​CTT​CAA​ACC​CAA​CT	159
		R	ACC​GGC​GAC​CAG​ATG​ATG​TA
Lipid metabolism	SREBF1	F	GCC​CTC​TGT​GCC​TTT​GTC​TTC	130	Hu et al. [12]
		R	ACT​CAG​CCA​TGA​TGC​TTC​TTC​C
Bile acid receptor	FXR	F	AGT​AGA​AGC​CAT​GTT​CCT​CCG​TT	182
		R	GCA​GTG​CAT​ATT​CCT​CCT​GTG​TC
Actin beta	ACTB	F	GCC​CCC​AAA​GTT​CTA​CAA​T	110	Rodríguez‐Hernández et al. [22]
		R	AGG​CGA​GTA​ACT​TCC​TGT​A