Prevalence of Ticks Infesting Dairy Cattle and the Pathogens They Harbour in Smallholder Farms in Peri-Urban Areas of Nairobi, Kenya

Figure 3

Maximum-likelihood tree of Ehrlichia spp. reconstructed based on partial sequences of 16S rDNA gene with 1000 bootstrap replicates. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 20 nucleotide sequences with five tick isolates (green dots) from this study and the other fifteen obtained from the GenBank. The tree indicates the phylogenetic relatedness of Ehrlichia isolates from ticks infesting cattle in peri-urban areas of Nairobi, Kenya, marked with green dots and sequences from other countries. Anaplasma phagocytophilum was used as an out-group. Sequence accession numbers are indicated at the end of each isolate.