Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Abaʼala District of Afar Region, Ethiopia

Table 1

The Universal primers and thermal cycle used for amplification of FMDV.

Primer-FMDV7 universal-forward 5pm/μl 5ʹ-GCCTGGTCTTTCCAGGTCT-3ʹ
Primer-FMDV7 universal-reverse-5pm/μl 5ʹ-CCAGTCCCCTTCTCAGATC-3ʹ
	Temperature	Time	Cycle
Initial denaturation	95	5 min	1 cycle
1^st denaturation	94	1 min	35 cycles
Annealing	54	1 min
Elongation	72	1 min
Final elongation	72	10 min	1 cycle