PCR-Based Direct Detection of Streptococcus uberis from Subclinical and Clinical Dairy Cattle Milk Samples

Table 1

Oligonucleotide primer sequences used in this study.

Designation	Target	Primer sequence	Product size	Annealing temp (°C)
665 Fwd; 666 rev	16 S rRNA; 16 S rRNA	5′-GAGAGTTTGATCCTGGCTCAGGA-3′; 5′-TTACCGCGGCTGCTGGCACGT-3′	529 bp	60
1100 Fwd; 1101 rev	S. uberis sub0888; S. uberis sub0888	5′-CTTTATGAAAATAGCCAAGCTGAAA-3′; 5′-TGTGAGCCAGTTGGAGGAAG-3′	974 bp	61
1102 Fwd; 1103 rev	S. uberis sub1154; S. uberis sub1154	5′-ACAAAGTTGAAAAGGGGCGT-3′; 5′-CGCCATTAGGTGAAAGTGCT-3′	573 bp	61