PCR-Based Direct Detection of Streptococcus uberis from Subclinical and Clinical Dairy Cattle Milk Samples

Figure 4

Detection of S. uberis from experimentally challenged cows in comparison to bacterial concentration and clinical score. (a) Comparison of bacterial cell counts obtained from direct plating of infected milk samples and detection of S. uberis from the same samples by multiplex PCR. (--) negative for sub0888 and sub1154, (−+) positive for sub1154 only, (++) positive for sub0888 and sub1154, with bacterial detection by PCR confirmed by 24 hrs after infection in all samples. (b) Clinical scores for milk quality and composition as per previous published guidelines (Field 2003).

(a)

(b)