Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines

Figure 1

MYOD1+ NOG+ cells predominant survival in RD and A-204 cell cultures after treatment with either Vincristine or Doxorubicin. (a) Plots show examples of flow analysis of RD and A-204 cells treated with Vincristine and Doxorubicin. In grey is highlighted the quadrant representing MYOD1+ NOG+ cells. (b) Percentage of live MYOD1+ NOG+ phenotype cells in RD and A-204 cell cultures after 2 days of treatment either with 1 and 10 nM of Vincristine (top panel) or with 0.1 and 1 μM of Doxorubicin (bottom panel). (c) Absolute numbers of total live cells in RD and A-204 cultures treated with 10 nM of Vincristine or 1 μM of Doxorubicin for 2 days. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change). Data are shown as mean ± standard deviation (N = 11). , , , ANOVA with Tukey's multiple comparison test.

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