The Fusion Oncogene FUS-CHOP Drives Sarcomagenesis of High-Grade Spindle Cell Sarcomas in Mice

Figure 4

p53 signaling and FUS-CHOP expression in FUS-CHOP-driven primary tumor cell lines. (a) Mouse background and tumor generation methods for primary tumors. (b) FUS-CHOP expression in primary mouse tumors and cell lines. (c) p53 and p21 expression 1 hour post-10 Gy irradiation in primary mouse tumors generated in different mouse backgrounds. 3T3 cells are mouse fusion negative controls, and 3T3-FC are mouse fusion positive controls for p53 signaling. (d) mRNA expression of p53 target genes 1 hour after treatment with 10 Gy ionizing radiation in NIH-3T3 and FUS-CHOP-driven tumor cell lines.