Using the Spleen as an In Vivo Systemic Immune Barometer Alongside Osteosarcoma Disease Progression and Immunotherapy with α-PD-L1

Table 5

Population distribution comparison of LLCs isolated from the spleens of mice from TF tumor-bearing (n = 5) and SH (n = 4) groups.

Population	TF % expression/TCES	SH % expression/TCES	p
NK cells	10.78 ± 4.5%	1.85 ± 0.4%	0.006
CTLs	2.73 ± 1.22%	3.72 ± 0.86%	0.21
Ths	6.45 ± 3.08%	7.72 ± 1.1%	0.46
Tregs	21.09 ± 5.12%	7.25 ± 0.23%	0.0011
CTLA-4^+ CTLs	18.35 ± 10.12%	1.72 ± 0.42%	0.014
TCES	18.23 ± 4.58	0.16 ± 0.054	0.0001

The statistics for each group are summarized accordingly with significance being assigned to those populations presenting values < 0.0083 from a two-tailed Student’s T-test with Bonferroni correction and 95% confidence as denoted by an asterisk. LLC = lymphoid lineage cell; TF = transfected; NK = natural killer; CTL = cytotoxic T lymphocyte; Th = T helper cell; Treg = T regulatory cell; CTLA-4⁺ CTL = cytotoxic T-lymphocyte-associated protein 4 positive cytotoxic T lymphocyte; TCES = T-cell exhaustion status.