Potential for Modulation of the Fas Apoptotic Pathway by Epidermal Growth Factor in Sarcomas

Table 5

Predictive value of EGF, TGFβ1, CTGF, and FasL for Fas, CASP8, and CASP10 mRNA concentrations in sarcomas and in serum-free (SF) and complete medium (CM) control and rhEGF-treated SW1353 chondrosarcoma cell cultures. Refer to Section 3.3 for separation of tumors into “top 41” and “bottom 41” tumor subcohorts. Significant probability values are in bold.

Samples	Response gene	Predictor genes
		EGF	TGFβ1	FasL	CTGF
82 tumors	Fas	0.11	0.09	5.0E − 03	1.8E − 04
Top 41 tumors	Fas	2.0E−03	0.48	9.0E − 04	0.01
Bottom 41 tumors	Fas	0.75	0.26	0.15	4.0E − 04
82 tumors	CASP8	0.21	0.03	0.06	0.94
Top 41 tumors	CASP8	0.34	3.5E − 03	9.3E − 03	0.96
Bottom 41 tumors	CASP8	0.13	0.89	0.35	0.50
82 tumors	CASP10	0.70	0.01	1.4E − 04	0.38
Top 41 tumors	CASP10	0.36	2.9E − 03	0.04	0.92
Bottom 41 tumors	CASP10	0.62	0.88	5.9E − 03	0.11
SW1353 SF control	Fas	0.31	7.4E − 05	n/a	0.22
SW1353 CM control	Fas	0.41	0.57	n/a	9.5E − 06
SW1353 rhEGF	Fas	0.24	6.8E − 09	n/a	0.33
SW1353 SF control	CASP8	0.23	0.47	n/a	0.25
SW1353 SF control	CASP10	0.09	0.20	n/a	0.19
SW1353 CM control	CASP8	0.50	2.9E − 03	n/a	2.8E − 03
SW1353 CM control	CASP10	0.80	1.1E − 03	n/a	3.6E − 04
SW1353 rhEGF	CASP8	6.3E − 07	0.28	n/a	0.70
SW1353 rhEGF	CASP10	1.0E − 06	0.09	n/a	0.29