GPA Peptide Attenuates Sepsis-Induced Acute Lung Injury in Mice via Inhibiting Oxidative Stress and Pyroptosis of Alveolar Macrophage

Figure 4

GPA peptide treatment inhibited caspase-1-dependent pyroptosis in vivo. (a) Flow cytometry analysis showing caspase-1 and PI staining. Pyroptotic cells from different groups are indicated as caspase-1⁺ and PI⁺. (b) Effects of GPA peptide on GSDMD expression. Expression of GSDMD in different cohorts was assessed using immunofluorescence. The . (c) Immunoblot analysis of the pore-forming mediator of pyroptosis. GPA peptide markedly decreased the development of the active, cleaved N-GSDMD protein in lung tissues of sepsis mice. Data are shown as the . vs. the sham cohort; ^### vs. the CLP cohort.

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