Research Article
Dioscin Improves Pyroptosis in LPS-Induced Mice Mastitis by Activating AMPK/Nrf2 and Inhibiting the NF-κB Signaling Pathway
Table 1
Primers used for qRT-PCR.
| | Gene | Sequence |
| | β-Actin | F: 5-GTCAGGTCATCACTATCGGCAAT-3 | | R: 5-AGAGGTCTTTACGGATGTCAACGT-3 |
| | TNF-α | F: 5-CCACGCTCTTCTGTCTACTG-3 | | R: 5-CCACGCTCTTCTGTCTACTG-3 |
| | IL-1β | F: 5-TGTGATGTTCCCATTAGAC-3 | | R: 5-AATACCACTTGTTGGCTTA-3 |
| | IL-6 | F: 5-AGCCACTGCCTTCCCTAC-3 | | R: 5-TTGCCATTGCACAACTCTT-3 |
| | INOS | F: 5-GAACTGTAGCACAGCACAGGAAAT-3 | | R: 5-CGTACCGGATGAGCTGTGAAT-3 |
| | COX-2 | F: 5-CGTACCGGATGAGCTGTGAAT-3 | | R: 5-CCAGCACTTCACCCATCAGTT-3 |
| | NLRP3 | F: 5-GAGCTGGACCTCAGTGACAATGC-3 | | R: 5-ACCAATGCGAGATCCTGACAACAC-3 |
| | ASC | F: 5-CAGGCGAGCAGCAGCAAGAG-3 | | R: 5-CAAGAGCGTCCAGGATGGCATC-3 |
| | Caspase-1 | F: 5-ACAACCACTCGTACACGTCTTGC-3 | | R: 5-CCAGATCCTCCAGCAGCAACTTC-3 |
| | Caspase-3 | F: 5-CGACTGGCGTGTGCGAGATG-3 | | R: 5-AGCAGCAGCAGCAGCAACAG-3 |
| | GSDMD | F: 5-AGTTCCGCTCTTGGTCGTG-3 | | R: 5-CTGCCGCTTACCTCCTTGAT-3 |
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