Dioscin Improves Pyroptosis in LPS-Induced Mice Mastitis by Activating AMPK/Nrf2 and Inhibiting the NF-κB Signaling Pathway

Figure 7

Dioscin inhibits activation of NF-κB signaling pathway and NLRP3 inflammasome in LPS-induced mice mastitis. The mRNA levels of (c) NLRP3, (e) ASC, (g) caspase-1, and (l) GSDMD were detected in mammary tissue homogenate. (a) Western blot analysis of (b) pp65, p65, (d) NLRP3, (f) ASC, (h) caspase-1, (i) caspase-3, and (k) GSDMD-N in mammary tissue homogenate. Relative protein levels of NLRP3, ASC, caspase-1, caspase-3, and GSDMD were normalized to β-actin. (j) Immunohistochemical staining for GSDMD; the scale bar represents 250 μm and 500 μm. ^# and ^## compared to the control group; and compared to the LPS+ATP group.

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