HSP60 Regulates Monosodium Urate Crystal-Induced Inflammation by Activating the TLR4-NF-κB-MyD88 Signaling Pathway and Disrupting Mitochondrial Function

Figure 3

HSP60 knockdown reduced the expression of MSU crystal-induced inflammatory associated gene in THP-1-derived macrophages. THP-1-derived macrophages were transfected with ctrl siRNA or HSP60 siRNA for 48 h, primed with LPS (100 ng/ml) for 1 h, and then stimulated with MSU crystals (50 μg/ml) for 12 h. (a) Quantification of RT-PCR analysis of IL-1β, IL-6, TNFα, COX-2, and iNOS mRNA expression. (b) The release of IL-1β, IL-6, TNFα, and PGE2 in the culture supernatant was measured by ELISA. (c) Western blot analysis of COX-2 and iNOS protein levels. Data are represented as the for three experiments. .

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