Inhibition of Mitochondrial ROS by MitoQ Alleviates White Matter Injury and Improves Outcomes after Intracerebral Haemorrhage in Mice

Figure 6

MitoQ decreased the Fecl₂ insult-induced accumulation of mitochondrial ROS and ATP deletion in OLI-neu cells. The level of mitochondrial ROS accumulation (red) in the OLI-neu cells was examined using MitoSox Red staining 24 hours after FeCl₂ (250 μM) treatment and treatment with or without NAC (1 mM) or MitoQ (200 μM). The staining was analyzed by microscopy (a) and flow cytometry (c). The data were analyzed in (b) and (d). (e) ATP content was analyzed using in OLI-neu cells after 24 hours of FeCl₂ treatment and treatment with or without NAC (1 mM) or MitoQ (200 μM). Scale bars in (a) and (c): 20 μm. The data are expressed as mean and SEM and were analyzed using 2-way ANOVA. represents Fe²⁺ versus control; ^### represents Fe²⁺ versus Fe²⁺+MitoQ; ^&, ^&&& represents Fe²⁺+NAC versus control.

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