Inhibition of Mitochondrial ROS by MitoQ Alleviates White Matter Injury and Improves Outcomes after Intracerebral Haemorrhage in Mice

Figure 5

MitoQ protected OLI-neu cells from mitochondrial membrane potential decrease and cell death caused by FeCl₂. (a) After 24 hours of treatment with FeCl₂ (250 μM) and cotreatment with or without NAC (1 mM) or MitoQ (200 μM), TMRM fluorescence was detected in OLI-neu cells by flow cytometry. (b) Quantitative analyses of TMRM fluorescence; . Scale bars: 20 μm. The data are expressed as means and SEM and were analyzed using ANOVA followed by Tukey’s post hoc test. (c) OLI-neu cells were treated with FeCl₂ and with or without NAC (1 mM) or MitoQ (200 μM). Cell death was detected by propodium iodide staining and flow cytometry after 48 hours. (d) Quantitative analyses of PI-positive cells, . The data are expressed as mean and SEM and were analyzed using 2-way ANOVA followed by Tukey’s multiple comparisons test. ; represents Fe²⁺ versus control; ^#, ^### represents Fe²⁺ versus Fe²⁺+MitoQ; ^&, ^&&& represents Fe²⁺+NAC versus control.

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