Protective Effect of Epigallocatechin-3-Gallate in Hydrogen Peroxide-Induced Oxidative Damage in Chicken Lymphocytes

Table 2

Primer sequences used for real-time qPCR assay.

Gene^†	Sequence (5-3)^‡	Product size (bp)
SOD	CGTCATTCACTTCGAGCAGAAGG	233
	GTCTGAGACTCAGACCACATA
HO-1	ACTTCTATGGCAGCAACT	129
	AATAGCGGGTGTAGGC
CAT	CTGTTGCTGGAGAATCTGGGTC	160
	TGGCTATGGATGAAGGATGGAA
GSH-PX	TTGTAAACATCAGGGGCAAA	140
	TGGGCCAAGATCTTTCTGTAA
Nrf2	ATTGAGCAAGTTTGGGAGGA	214
	AAGACACTGTAACTCAGGAATGGA
Trx-1	GCAGGACAGGCTGGAACTCACA	152
	CGAGAAGTGCGAGGTGAACG
β-Actin	GAGAAATTGTGCGTGACATCA	152
	CCTGAACCTCTCATTGCCA

^†SOD: superoxide dismutase; HO-1: Heme oxygenase-1; CAT: catalase; GSH-PX: glutathione peroxidase; Nrf2: nuclear factor erythroid 2-related factor 2; Trx-1: thioredoxin-1. ^‡Shown as forward primer followed by reverse primer.