Research Article
Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway
Figure 13
Melatonin effects on the autophagy of spermatogonial stem cells (SSCs) during cryopreservation. (a) Autophagosomes in goat SSCs were characterized by transmission electron microscopy (TEM). (b) The number of autophagosomes. (c) Western blot image of LC3-I, LC3-II, P62, BECN1, and ATG7. (d–i) Relative protein expression in the control group and 10-6 M melatonin treatment group. The superscript indicates a significant difference at , and the superscript indicates a significant difference at . The superscript ns indicates no significant difference. Values are expressed as the (). Mel: 10-6 M melatonin. .
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