Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway

Figure 12

Mitochondrial status of frozen-thawed goat spermatogonial stem cells (SSCs). (a) The mitochondrial swelling and vacuolation in goat SSCs were characterized by transmission electron microscopy (TEM). The yellow arrows indicate swollen and vacuolated mitochondria, and the white arrow indicates normal mitochondria. (b) Statistical analysis results of mitochondrial swelling and vacuolation. (c) Membrane blebbing. (d) Nuclear change. (e) Western blot image of cytochrome C in the cytoplasm and mitochondria. (f, g) Relative protein expression in the control and 10^-6 M melatonin treatment group. The superscript indicates a significant difference at , and the superscript indicates a significant difference at . Values are expressed as the (). Mel: 10^-6 M melatonin. .

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