Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins

Figure 2

Differentially expressed hippocampal and cortical proteins in aged vs. young mice identified by NanoLC-ESI-MS/MS. (a and b) Proteins differentially expressed in 390 hippocampi and 258 cerebral cortices were hierarchically clustered for 4-month and 16-month-old mice. Differential expression was defined as at least 1.2 times (increased) or ≤0.8-fold (decreased) expression in aged vs. young animal brain. The color of each cell represents the expression level of the protein: red signifies an increase and blue a decreased level relative to that of the control group (4-month-old mice). /6 for per group. Identified proteins are primarily involved in mitochondrial dysfunction, synaptic dysfunction, oxidative stress, ribosome, cytoskeletal integrity, transcriptional regulation, and GTPase function. (c) The Venn logic diagram between the dysregulated proteins of the hippocampus and cerebral cortex in 4-month and 16-month-old mice.

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