Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins

Figure 1

Experimental flowchart. We employed 4-month-old and 16-month-old mice, respectively, each group of 10 mice. From each group, hippocampal and cerebral cortical tissues of 5 or 6 animals were randomly selected for proteomic analysis. For each group, proteins were extracted from 5 or 6 individual samples and pooled (1 : 1 : 1 : 1 : 1 or 1 : 1 : 1 : 1 : 1 : 1) for subsequent proteomic experiments. The pooled proteins were digested into peptides using trypsin/Lys-C Mix and subjected to 4-plex TMT labeling, High pH reversed-phase peptide fractionation and subsequent NanoLC-ESI-MS/MS analysis. The raw data were identified and quantified using PEAKS 8.5 software and subjected to bioinformatics analysis (GO, KEGG, STRING, and Wiki pathways). Selected key proteins were verified by Western blot.