Research Article
Danhong Injection Alleviates Cardiac Fibrosis via Preventing the Hypermethylation of Rasal1 and Rassf1 in TAC Mice
Figure 7
DHI treatment regulates expressions of Rasal1 and Rassf1 and reduced the hyperactivity of Ras/ERK in CF hearts. (a) Quantification of RASAL1 protein level in CF hearts. (b) Quantification of RASSF1 protein level in CF hearts. (c) Quantification of TGF-β1 protein level in CF hearts. (d) Quantification of α-SMA protein level in CF hearts. (e) Representative Western blots of RASAL1, RASSF1, TGF-β1, α-SMA, and GAPDH. (f) Relative mRNA level of Rasal1 in CF hearts. (g) Relative mRNA level of Rassf1 in CF hearts. (h) Relative mRNA level of TGF-β1 in CF hearts. (i) Relative mRNA level of Acta2 (α-SMA) in CF hearts. (j) Phosphorylated level of ERK in CF hearts. (k) Representative Western blots of phosphorylated and total ERK. . .
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