Danhong Injection Alleviates Cardiac Fibrosis via Preventing the Hypermethylation of Rasal1 and Rassf1 in TAC Mice

Figure 6

Bisulfite sequencing results of the Rasal1 and Rassf1 gene clusters. (a) Alignment results of bisulfite sequencing reads in the Rasal1 gene cluster are shown in the UCSC genome browser. (b) Alignment results of bisulfite sequencing in the Rassf1 gene cluster are illustrated in the UCSC genome browser. (c) The bisulfite PCR products corresponding to the region in the Rasal1 gene shown in (a) were analyzed using QUMA. (d) The bisulfite PCR products corresponding to the region in the Rassf1 gene shown in (b) were analyzed using QUMA. Each circle indicates a CpG site, and the methylated site is indicated in black. (e) QUMA analysis of the Rasal1 gene is shown as a bar graph. (f) QUMA analysis of the Rassf1 gene is displayed as a bar graph. Each bar represents the average methylation level of all CpG sites analyzed by each bisulfite product. Error bar is generated from 3 replicates. .