Danhong Injection Alleviates Cardiac Fibrosis via Preventing the Hypermethylation of Rasal1 and Rassf1 in TAC Mice

Figure 3

DNA methylation changes induced by TAC. (a) Methylation sites in genetic annotation of TAC mice. (b) Methylation sites in genetic annotation of sham-operated mice. (c) The total methylation levels of heart samples were compared between TAC (C1, C2, C3, and C4) and sham-operated (S1, S2, S3, and S4) groups. (d) The average methylation levels in landscape regions were compared between TAC and sham-operated groups (). (e) DNA methylation differences, including 19,618 hypermethylated genes and 22,988 hypomethylated genes, were detected between TAC and sham-operated groups. (f) PCA was performed to identify the clustering profiles of the methylated alterations induced by TAC. The red circle clustered samples from TAC mice (C1, C2, C3, and C4), and the blue circle clustered samples from sham-operated mice (S1, S2, S3, and S4).

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