NAMPT/SIRT1 Attenuate Ang II-Induced Vascular Remodeling and Vulnerability to Hypertension by Inhibiting the ROS/MAPK Pathway

Figure 7

The MAPK signaling pathway is responsible for the antihypertensive effects of NAMPT/SIRT1. Western blots showing the levels of P-ERK1/2, P-JNK1/2, and P-P38 and quantification relative to the total ERK1/2, JNK1/2, and P38 levels in the MAECs (a) and MOVAs (b) transfected with the SIRT1-specific siRNA and NAMPT overexpression plasmid and stimulated with Ang II for 48 h. (c) Representative Western blots and quantitative analyses of the levels of the P-ERK1/2/ERK1/2, P-JNK1/2/JNK1/2, and P-P38/P38 proteins in fresh aortic wall tissues obtained from NAMPT+/- and WT mice after Ang II stimulation ( in each group). Data are presented as . , , , and NS indicates no significant difference between the 2 indicated groups.

(a)

(b)

(c)