NAMPT/SIRT1 Attenuate Ang II-Induced Vascular Remodeling and Vulnerability to Hypertension by Inhibiting the ROS/MAPK Pathway

Figure 5

NAMPT alleviates Ang II-induced endothelial injury and changes in the proliferation and migration of MOVAs by targeting NAD/SIRT1 signaling. (a) The protein levels of apoptosis markers (Caspase 3 and Bcl-2) and P-eNOS/eNOS in NAMPT-overexpressing cells transfected with SIRT1-specific siRNA and stimulated with Ang II for 48 h were determined using Western blot analysis; the densitometric quantification is also shown. (b) The expression of the endothelial cell permeability marker ZO-1 in MAECs was determined using immunofluorescence staining (ZO-1: red; nuclei (DAPI): blue). Scale  μm. (c) MAECs subjected to different treatments were stained with Annexin V and PI and analyzed using flow cytometry to evaluate the effect of NAMPT on cell apoptosis after the Ang II treatment. Quantification of the cell apoptosis rate is shown in the right panel. (d) Relative levels of proliferation-related proteins (PCNA and cyclin D1) and extracellular matrix synthesis-related proteins (MMP-2 and MMP-9) in cultured MOVAs from the indicated groups. (e, f) Representative images of scratch-wound assays and transwell assays and quantification of migrated MOVAs ( in each group; scale b μm). Data are presented as ., , and compared with the Ctrl or between the 2 indicated groups.

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