NAMPT/SIRT1 Attenuate Ang II-Induced Vascular Remodeling and Vulnerability to Hypertension by Inhibiting the ROS/MAPK Pathway

Figure 4

NAMPT regulates SIRT1 expression in vivo and in vitro. Representative qRT-PCR results and summarized data showing the expression of SIRT1-7 in MAECs (a) and MOVAs (c) transfected with NAMPT and stimulated with Ang II. Representative Western blot and summarized data showing the levels of SIRT1 in MAECs (b) and MOVAs (d) transfected with NAMPT plasmids and stimulated with Ang II. (e) Representative qRT-PCR and summarized data showing the expression of SIRT1-7 from NAMPT haplodeficient (NAMPT+/-) mice and their littermate control mice after Ang II stimulation ( in each group). (f) Representative Western blot and quantitative analysis of SIRT-1 levels among the different groups. (g) Immunohistochemical staining showing the level of SIRT1 in Ang II-treated NAMPT haplodeficient (NAMPT+/-) mice and their littermate control mice ( in each group; scale bar 50 μm). Data are presented as . , , and , and NS indicates no significant difference between the 2 indicated groups.

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