Peroxiredoxin 6 Is a Key Antioxidant Enzyme in Modulating the Link between Glycemic and Lipogenic Metabolism

Figure 2

PRDX6^-/- mice display impaired glucose tolerance and insulin sensitivity following 24 weeks on HFD. (a) After an overnight fast, an IPGTT was performed by injecting intraperitoneally 2 g/kg body weight of D-glucose. Blood samples were taken from the retroorbital cavity of the mice at 0, 15, 30, 60, 90, and 120 minutes after the injection of D-glucose. WT mice (open circle, solid line) and PRDX6^-/- mice (filled triangle, dashed line) ( mice per group). (b) ITT was performed by intraperitoneal injection of insulin at 0.75 IU/kg body weight following 4 hours of fasting. After the injection of insulin, blood glycaemia was measured at 0, 15, 30, and 60 minutes. WT mice (open circle, solid line) and PRDX6^-/- mice (filled triangle, dashed line) ( mice per group). (c) The quantitative assessment of plasma insulin secretion during the IPGTT were analyzed by ELISA at 0, 15, 60, and 120 minutes after glucose injection. WT mice (open circle, solid line) and PRDX6^-/- mice (filled triangle, dashed line) ( mice per group). (d) Calculation of insulinogenic index as delta insulin to the delta glucose ratio (/) in WT (black bar) and PRDX6^-/- mice (white bar) ( mice per group). (e and f) Valuation of islet density (e) and size (f) in WT mice (black bars) and PRDX6^-/- mice (white bar). (g and h) Representative microphotographs of pancreatic islet of Langerhans after 24 weeks on HFD in WT mice (g) and PRDX6^-/- mice (h). The images are shown at 20x magnification. The results are expressed as . , , and .

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