ALCAT1 Overexpression Affects Supercomplex Formation and Increases ROS in Respiring Mitochondria

Figure 5

Mitochondrial ROS levels in ALCAT1 overexpressing cells under different nutrition conditions. (a) Dual color images showing ROS formation in cells with normal ALCAT1 levels and cells with artificially elevated ALCAT1 levels (tr. ALCAT1: transiently transfected cells expressing ALCAT1-2A-GFP-NLS). The transfected cells were identified by their green nuclei (GFP::NLS). Cells were supplied with different sugars as indicated before for 48 h. After staining with MitoTracker Red, cells were analyzed with a fluorescence microscope (MitoTracker Red: 561 nm, 600-700 nm, GFP: 488 nm, 510-540 nm). (b) Quantitative analysis of ROS formation as MitoTracker Red fluorescence intensity signal (arbitrary units, a.u.). Results are presented as box plots with the measured fluorescence intensities of ~45 measured cells. Data was analyzed by One-Way ANOVA with post hoc Scheffe (; ; and ), SD are shown.

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