ALCAT1 Overexpression Affects Supercomplex Formation and Increases ROS in Respiring Mitochondria

Figure 4

Effects of elevated ALCAT1 levels on mitochondrial bioenergetics. Automatic flux analysis of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of HeLa WT supplied with glucose and galactose for 48 h and ALCAT1 overexpressing cells under the same conditions. (a) Oxygen consumption rate (OCR) of HeLa WT and transiently transfected ALCAT1 overexpressing cells. OCRs (and ECAR) were analyzed with the Seahorse XF Cell Mito Stress Test Kit by sequential injection of oligomycin (2 μM), FCCP (1 μM), and rotenone/antimycin A (0.5 μM). The cells were supplied with 5.6 mM glucose or 10 mM galactose for 48 h before measurement. replicates with 30 wells for each condition. (b) Basal and maximal respiration, spare respiratory capacity, CV-linked respiration, proton leak, and nonmitochondrial respiration determined from OCRs shown in (a). One Way ANOVA with post hoc Scheffe (; ; and ). (c) Corresponding extracellular acidification rates (ECAR).

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