ALCAT1 Overexpression Affects Supercomplex Formation and Increases ROS in Respiring Mitochondria

Figure 3

Analysis of mitochondrial morphology in ALCAT1 overexpressing cells. Transiently transfected ALCAT1-2A-GFP-NLS (tr. ALCAT1) and HeLa WT cells were supplied with 5.6 mM glucose and 10 mM galactose, respectively, for 48 h. Quantitative analysis of mitochondrial network morphology by the ImageJ™ plugin MiNA. MitoTracker Red stained mitochondria were analyzed. Morphological data are extracted from the MiNA-processed (= contrast enhanced) images. (a) MiNa-processed images (mitochondrial skeleton in red) of HeLa cells after MitoTracker Red staining of HeLa WT and ALCAT1 overexpressing cells in Glc and Gal (scale bars: 10 μm). (b) Quantitative MiNA analysis of mean branch length of mitochondria. (c) Quantitative MiNA analysis of mitochondrial footprint. The footprint is simply the sum of positive pixels per cell and represents the mitochondrial area. biological replicates. Each data point represents data from one cell. Statistics: data was analyzed by One-Way ANOVA with post hoc Fisher LSD (; ; and ), SD are shown.

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