N^ω-(Carboxymethyl)arginine Is One of the Dominant Advanced Glycation End Products in Glycated Collagens and Mouse Tissues

Figure 1

Measurement of CMA in glycated proteins by ESI-LC-MS/MS. (a) 13C in [¹³C₆] CMA is indicated by an asterisk. Type I collagen and HSA were incubated with glucose at 37°C for up to 4 weeks. The parent ions of CMA and [¹³C₆] CMA were 233 () and 239 (), respectively, and peaks of the fragment ion of 116 () for CMA (b) and 121 () for [¹³C₆] CMA (c) were detected in the samples of glycated collagen. The amounts of CMA (d) and CML (e) were normalized by the amounts of proteins in glycated proteins. The amount of CMA (f) was normalized by the arginine content, and CML (g) was normalized by the lysine content.

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