Research Article
lncRNA AK054386 Functions as a ceRNA to Sequester miR-199 and Induce Sustained Endoplasmic Reticulum Stress in Hepatic Reperfusion Injury
Figure 4
AK054386 affects hepatic injury from ischemia and reperfusion in vivo and in vitro. (a) AK054386 impacts apoptosis in the BNL-CL2 IRI cell model. Apoptosis rates were assayed by flow cytometry. Representative data from three independent experiments are shown. (b) BNL-CL2 cell death levels were assayed by LDH release. Data are shown as the of three independent experiments. (c, d) The protein levels of ER-related genes were analyzed by Western blot. (e) The relative RNA expression levels of inflammatory cytokines (IL-6 and TNF-α) of liver tissues from mouse hepatic IRI models after lentivirus infection. 5 mice were analyzed in each group. The RNA levels were measured by qRT-PCR and normalized to GAPDH. Data are shown as the of three independent experiments. ANOVA, and . (f, g) Mouse serum ALT and AST levels were analyzed and presented as the Five mice were analyzed in each group. ANOVA, and . (h, i) Representative light photomicrographs of TUNEL-stained sections of mouse liver tissue from control mice and mouse hepatic IRI models after lentivirus infection. Five mice were analyzed in each group.
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