Obeticholic Acid Protects against Gestational Cholestasis-Induced Fetal Intrauterine Growth Restriction in Mice

<div>OCA inhibited the upregulation of placental NADPH oxidase-4 and antioxidant genes during E2-induced cholestasis. All pregnant mice except controls were s.c. injected with E2 (0.625 mg/kg) once daily from GD13 to GD17. In the OCA+E2 groups, pregnant mice were administered with OCA (5 mg/kg) by gavage once daily from GD12 to GD17. All dams were sacrificed on GD18. (a, b) Placental NADPH oxidase 4 (NOX4) was measured using immunoblot. (a) Representative gels were shown. (b) NOX4/<i>β</i>-actin. (c, d) The expressions of placental peroxiredoxins (<i>prdx</i>) were measured using real-time RT-PCR: (c) <i>prdx1</i> mRNA and (d) <i>prdx3</i> mRNA. 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Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: Obeticholic Acid Protects against Gestational Cholestasis-Induced Fetal Intrauterine Growth Restriction in Mice 