The Ameliorative Effects of the Ethyl Acetate Extract of Salicornia europaea L. and Its Bioactive Candidate, Irilin B, on LPS-Induced Microglial Inflammation and MPTP-Intoxicated PD-Like Mouse Model

Figure 2

Bioactivity-guided isolation and characterization of Irilin-B from SE-EA fraction. Antioxidant activity-guided isolation and characterization of compound L13-1 from SE-EA. (a) Purification scheme of compound L13-1 from SE-EA; (b) representative HPLC profiles of SE-EA, SE-EA-L13, and finally purified compound L13-1, Irilin B. HPLC (1260 Infinity, Agilent Technologies, Santa Clara, CA, USA) equipped with a ZORBAX Eclipse XDB C18 prep column (, 5 μm, Agilent Technologies) was conducted with a gradient eluent of methanol and 0.04% trifluoroacetic acid as the mobile phase. The UV spectrum and chemical structure of the finally purified compound L13-1 are also depicted. (c) NMR spectra of the purified compound L13-1. c1: ¹H-NMR spectrum; c2: ¹³C-NMR spectrum. (d) Determination of stereographic structure by analysis of two-dimensional ¹H-¹H COSY and HMBC-NMR spectra and assignments of carbon and hydrogen in NMR spectra.

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