Phosphocreatine Improves Cardiac Dysfunction by Normalizing Mitochondrial Respiratory Function through JAK2/STAT3 Signaling Pathway In Vivo and In Vitro

Figure 6

PCr inhibits MGO-induced apoptosis by modulating Bax, Bcl-2, caspase 3, caspase 9, cleaved caspase 3, cleaved caspase 9, and cytochrome c (mitochondria) in H9C2 cells. (a) Cells were treated with PCr (5~20 mM) for 2 h preceding being presented to 1 mM MGO for 24 h. Cell apoptosis was estimated by flow cytometry. (b) Apoptotic cells represent the percentage of Annexin V single positive and Annexin V/PI twofold positive cells. (c) The protein dimensions of Bcl-2, Bax, caspase 3, caspase 9, cleaved caspase 3, cleaved caspase 9, and cytochrome c (mitochondria) were recognized by western blot in cells. (d, e, f, g, h, i, j, k) Quantifications of western blot. Data are exhibited as the (). , , and vs. the control group. , , and vs. the MGO group.

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