Cicadidae Periostracum, the Cast-Off Skin of Cicada, Protects Dopaminergic Neurons in a Model of Parkinson’s Disease

Figure 9

Effects of CP on LPS-induced neuroinflammatory signaling factors. Microglial BV2 cells were treated with CP for 2 h and with LPS for an additional 22 h. After 24 h incubation, the cultures were subjected to a cytokine antibody array assay (h). Densitometric ratios of the arrays showed differences in the cytokine markers (ICAM-1, KC, IL-6, MCP-5, and RANTES) (i–m). The glial activation markers (GFAP and Iba-1) and neuroinflammatory signaling factors (iNOS and Cox-2) were measured using western blotting (n). Bar graphs represent the relative expression of GFAP, Iba-1, iNOS, and Cox-2 (o) for (n). Values shown represent . , , and compared with the control group and , , and compared with the LPS-treated group.

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