Pioglitazone Protects Compression-Mediated Apoptosis in Nucleus Pulposus Mesenchymal Stem Cells by Suppressing Oxidative Stress

<div>Pioglitazone protected against compression-induced cytotoxicity in NP-MSCs. (a) Rhodamine-phalloidin was used to detect the actin filaments of NP-MSCs. The red fluorescence showed the actin (1000x). (b) The histogram shows the release of LDH. (c) Calcein-AM/PI dye was used to evaluate NP-MSCs damage under a fluorescence microscope. The green fluorescence indicates live cells, and the red fluorescence indicates dead cells (100x). (d) Quantitative analysis of calcein-AM-positive cells. (e) Quantitative analysis of PI-positive cells. 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Oxidative Medicine and Cellular Longevity

fig3

Figure 3

Figure 3: Pioglitazone Protects Compression-Mediated Apoptosis in Nucleus Pulposus Mesenchymal Stem Cells by Suppressing Oxidative Stress 