Pioglitazone Protects Compression-Mediated Apoptosis in Nucleus Pulposus Mesenchymal Stem Cells by Suppressing Oxidative Stress

Figure 1

Identification of NP-MSCs. (a) The growth state and morphology of the cells were observed under an optical microscope (40x and 100x). (b) A colony-forming assay was used to observe NP-MSCs-formed homogeneous colonies (1x). (c) Flow cytometry was used to detect the surface markers on the cells, including CD34, CD73, CD90, CD105, and HLA-DR. Quantitative analysis of positive cells in NP-MSCs. (d) Alizarin Red staining, Oil Red O staining, and Alcian blue were used to evaluate osteogenic, adipogenic, and chondrogenic differentiation of NP-MSCs, respectively (100x).

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