Autophagy Deficiency Leads to Impaired Antioxidant Defense via p62-FOXO1/3 Axis

Figure 4

Accumulated p62 in a condition of inhibited autophagy is responsible for the loss of FOXO1/3, which is involved in aging but not dominant in type 2 diabetes mellitus animal models. (a and b) HEK293T cells were transfected with sip62 or negative control siRNA or with sip62 and/or siAtg5 for 48 hours before harvest; indicated protein levels were determined by W.B. (c and d) Liver tissues from SD rats aged five months (young) and 25 months (old) were homogenized to yield lysates, protein carbonylation levels were detected with a commercially available kit, Coomassie brilliant blue staining was used to monitor equal loading control (c), and indicated protein levels were determined by W.B. and densitometry analysis () (d). (e and f) Liver tissues from 18-week-old ob/ob (B6/JNju-Lep^em1Cd25/Nju), db/db (BSK-Lepr^em2Cd479/Nju), and C57 mice were homogenized to yield lysates, indicated protein levels were determined by W.B. and densitometry analysis () (e), and ROS levels were measured with a DCFDA probe () (f). Values are represented as , and statistical analysis were conducted using -test. ; ; ; vs. young or C57.

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