Crosstalk between Mitochondrial Ca²⁺ Uptake and Autophagy in Skeletal Muscle

Figure 3

Autophagy flux analyses in MCU overexpressing muscles. (a-c) Immunoblotting analysis of EDL muscles infected with AAV-MCU or AAV-LacZ as control and treated or not with colchicine in fed conditions. Western blot analyses demonstrated efficient MCU overexpression in EDL muscles. Protein levels of LC3-II and p62 were used to monitor autophagy, relative to actin protein levels used as loading control. (b, c) Quantification by densitometry of the ratio between LC3-II/actin and p62/actin. , test (two-tailed, unpaired) of, respectively, three vehicle animals and four treated animals. Data are presented as . (d-f) Immunoblotting analysis of EDL muscles infected with AAV-MCU or AAV-LacZ as control and treated or not with colchicine upon starvation. Western blot analyses demonstrated efficient MCU overexpression in EDL muscles. (e, f) Quantification by densitometry of the ratio between LC3-II/actin and p62/actin. , test (two-tailed, unpaired) of four animals per condition. Data are presented as .