Crosstalk between Mitochondrial Ca²⁺ Uptake and Autophagy in Skeletal Muscle

Figure 1

Autophagy flux analyses in MCU-silenced muscles. (a-f) Immunoblotting analysis of EDL muscles infected with AAV-shMCU or AAV-shLUC as control and treated or not with leupeptin (a-c) or colchicine (d-f) in fed conditions. Western blot analyses demonstrated efficient MCU downregulation in EDL muscles. Protein levels of LC3-II and p62 were used to monitor autophagy, relative to actin protein levels used as loading control. (b, c, e, and f) Quantification by densitometry of the ratio between LC3-II/actin and p62/actin. , test (two-tailed, unpaired) of four animals per condition. Data are presented as . (g-l) Immunoblotting analysis of EDL muscles infected with AAV-shMCU or AAV-shLUC as control and treated or not with leupeptin (g-i) or colchicine (j-l) upon starvation. Western blot analyses demonstrated efficient MCU downregulation in EDL muscles. (h, i, k, and l) Quantification by densitometry of the ratio between LC3-II/actin and p62/actin. , test (two-tailed, unpaired) of four animals per condition. Data are presented as .