Attenuation of High-Fat Diet-Induced Rat Liver Oxidative Stress and Steatosis by Combined Hydroxytyrosol- (HT-) Eicosapentaenoic Acid Supplementation Mainly Relies on HT

Table 1

Gene-specific primer sequences used in the study.

mRNA	Forward primer	Reverse primer
Nrf2	AAGCTTTCAACCCGAAGCAC	TTTCCGAGTCACTGAACCCA
Gst	TGCAGACCAAAGCCATTCTC	ACGGTTCCTGGTTTGTTCCT
Ggt	ATGTGGACACCCGATGCAGTATT	TGTCTTGCTTGTAGTCAGGATGGTTT
β-Actin	ACTGCCGCATCCTCTTCCTC	CTCCTGCTTGCTGATCCACATC

Sequences are listed in the 5 → 3 direction. Nrf2: nuclear factor erythroid 2-related factor 2; Gst: glutathione-S-transferase; Ggt: γ-glutamyl transpeptidase.