Attenuation of High-Fat Diet-Induced Rat Liver Oxidative Stress and Steatosis by Combined Hydroxytyrosol- (HT-) Eicosapentaenoic Acid Supplementation Mainly Relies on HT

Figure 1

Liver histological assessment in mice subjected to control diet (CD) and high-fat diet (HFD) without and with eicosapentaenoic acid (EPA), hydroxytyrosol (HT), and EPA + HT supplementation. Representative liver sections from animals given (a) CD, (b) CD + EPA, (c) CD + HT, (d) CD + EPA + HT, (e) HFD, (f) HFD + EPA, (g) HFD + HT, and (h) HFD + EPA + HT (haematoxylin-eosin liver sections from 8 animals per experimental group; original magnification ×40). (i) Liver steatosis scores [24] expressed as for 8 animals per experimental group. Groups sharing the same symbol are not significantly different among them according to two-way ANOVA and the Bonferroni posttest (). ^,, and indicate the significant differences between the experimental groups.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)