Autophagy Stimulus Promotes Early HuR Protein Activation and p62/SQSTM1 Protein Synthesis in ARPE-19 Cells by Triggering Erk1/2, p38^MAPK, and JNK Kinase Pathways

Figure 2

Evaluation of p62 protein level following AICAR + MG132 exposure. (a) Representative Western blotting (upper) and densitometric analysis (lower) of p62 protein levels in the nucleus (a), cytoplasm (b), and total homogenate (c) of ARPE-19 cells exposed to either solvent (CTR) or AICAR + MG132 (A + M) for increasing times (15, 30, and 120 min). Optical densities of p62 bands were normalized to lamin C for the nucleus and to α-tubulin for both the cytoplasm and total homogenate. The same proteins were also used as purity controls for each cellular fraction. The values are expressed as mean percentages + S.E.M. (, ; Dunnett’s multiple comparison test).

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