Di-2-pyridylhydrazone Dithiocarbamate Butyric Acid Ester Exerted Its Proliferative Inhibition against Gastric Cell via ROS-Mediated Apoptosis and Autophagy

Figure 8

DpdtbA-induced autophagy in MGC-803 cells. Stained by acridine orange: (a) control, (b) 2.5 μM DpdtbA, (c) 5 μM DpdtbA, (d) 2.5 μM DpdtbA + 3-MA (2 mM), and (e) 5 μM DpdtbA + 3-MA (2 mM). (f) Western blotting: 1 = 5 μM DpdtbA plus 1.5 μM NAC; 2 = 5 μM DpdtbA; 3 = 5 μM DpdtbA plus 1.5 mM 3-MA; 4 = DMSO control; 5 = 1.5 mM NAC; 6 = 1.5 mM 3-MA.

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