FluoroGold-Labeled Organotypic Retinal Explant Culture for Neurotoxicity Screening Studies

Figure 5

FLOREC safety study. (a-b) The absolute LDH activity in culture medium of FLOREC explants. The gentamicin insult introduced on the third day of culture resulted in accelerated release of LDH which was higher in explants without CNTF treatment. (c–f) FG-labeled whole-mounted explants representing study groups (after 7 days of culture). (g) RGC count in FLOREC explants after 7 days of culture. ANOVA value for multiple comparisons = 0.001. Bolded italic values represent significance after post hoc Bonferroni correction. (h–k) Immunofluorescent staining of whole-mounted explants for β3tubulin (after 7 days of culture). (l) The correlation between LDH activity in culture medium and RGC count after 7 days of explant culture.

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