FluoroGold-Labeled Organotypic Retinal Explant Culture for Neurotoxicity Screening Studies

Figure 4

FLOREC/OREC comparative safety study. (a) The pH values of culture medium in OREC and FLOREC explants. (b) The absolute values of LDH activity in culture medium for FLOREC explants were slightly higher than for OREC explants without FG. (c-d) In explants without gentamicin insult (c), the LDH activity tended to decrease in time, while in case of explants after gentamicin exposure (d), there was rapid LDH outflux detected in culture medium due to cell membrane damage. The gentamicin insult was visibly alleviated by CNTF supplementation and resulted in almost 3-fold lower release of LDH. (e-f) Relative ratio comparisons of LDH activity. ANOVA value for multiple comparisons = 0.0001. Bolded italic values represent significance after post hoc Bonferroni correction.

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