FluoroGold-Labeled Organotypic Retinal Explant Culture for Neurotoxicity Screening Studies

Figure 3

The system validation group. (a–c) Comparison of waste medium pH, medium LDH activity, LDH activity change between day 7 and day 3 (∆LDH), and RGC count for FG and β3tubulin in OREC and FLOREC explants after 7 days of culture. In FLOREC explants, the difference in number of cells labeled with FG and β3tubulin was not significant, similarly as the difference in number of β3tubulin cells in FLOREC and OREC explants. nFLOREC = 16 explants, nOREC = 16 explants. (d–g) Immunofluorescent staining of whole-mounted explants for β3tubulin after 7 days of culture. (h-i) FG-labeled whole-mounted FLOREC explants after 7 days of culture.

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